Mouse IL-12(p70) ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0235
76237-150EA 760.6 USD
76237-150
Mouse IL-12(p70) ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Mouse Interleukin-12(p70) AccuSignal ELISA Kit


  • Natural and recombinant mouse IL-12(P70).
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: SF21; Immunogen sequence: (M23-S419)+(R23-A215)


Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse IL-12(P70) standard solution and each sample be measured in duplicate.

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