Rat CNTF ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0155
76237-122EA 760.6 USD
76237-122
Rat CNTF ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Rat CNTF AccuSignal ELISA Kit


  • Natural and recombinant rat CNTF.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: E.coli; Immunogen sequence: A2-M200


Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat CNTF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR