QuantiChrom™ Protein Creatinine Ratio Assay Kit, BioAssay Systems
Supplier: BIOASSAY SYSTEMS
For quantitative determination of urinary protein and creatinine concentrations (protein or creatinine ratio, UPCR).
- Sensitive and accurate, fast and convenient
- Use 20 µl samples. Linear detection range in 96-well plate: 1 to 20 mg/dl Protein and 1 to 150 mg/dL Creatinine
- No sample pre-treatment is needed, simple 10- minute "add-incubate-read" procedure
- High-throughput adaptable
- The procedure can be readily automated for processing thousands of samples per day
- Method: OD600 and OD530 nm
- Samples: Urine samples
Protein is filtered out of urine by the glomeruli of the kidneys. Albumin is the most common serum protein, thus the majority of the protein in urine is albumin. A damaged kidney will allow some protein through into the urine, the less protein in urine the better. Elevated protein levels in urine is called microalbuminuria or proteinuria, which typically arises due to type 1 diabetes, type 2 diabetes, or high blood pressure. Creatinine is synthesized in the body at a fairly constant rate from creatine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. Protein/creatinine ratio (PCR) remains the simplest and most convenient test for proteinuria. Other methods such as 24 hour urine test or timed urine test require strict adherence to sample collection protocol. Since the protein concentration is normalized to creatinine secretion, the urine sample can be taken at anytime and no diet or liquid restrictions are necessary for sample collection.
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