Mouse IL-23 ELISA Kit, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Mouse Interleukin-23 AccuSignal ELISA Kit
- Natural and recombinant mouse IL-23.
- There is no detectable cross-reactivity with other relevant proteins.
- Expression system for standard: sf21; Immunogen sequence: (M23-S419)+(L20-A196)
Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812).
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.
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