Rat VEGF ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0330
76236-822EA 760.6 USD
76236-822
Rat VEGF ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Rat VEGF AccuSignal ELISA Kit


  • Natural and recombinant rat VEGF.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: A27-R190


Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml rat VEGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each rat VEGF standard solution and each sample is measured in duplicate.

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