Human TNFsR I ELISA Kit, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Human TNFsR I AccuSignal ELISA Kit
- Natural and recombinant human TNFsR.
- There is no detectable cross-reactivity with other relevant proteins.
- Expression system for standard: NSO; Immunogen sequence: L30-T211
TNFsRI(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human TNFsRⅠ standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. We recommend that each human TNF s RⅠ standard solution and each sample is measured in duplicate.
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