Human M-CSF ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0253
76236-718EA 760.6 USD
76236-718
Human M-CSF ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human M-CSF AccuSignal ELISA Kit


  • Natural and recombinant human M-CSF.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: E.coli; Immunogen sequence:  E33-S190


M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human M-CSF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, tissue lysates, serum, plasma (heparin or EDTA), saliva or urine to each empty well. It is recommended that each human M-CSF standard solution and each sample be measured in duplicate.

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