Rat IL-4 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0222
76236-668EA 760.6 USD
76236-668
Rat IL-4 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Rat Interleukin-4 AccuSignal ELISA Kit


  • Natural and recombinant rat IL-4.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: E.coli; Immunogen sequence: C25-S147


Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat IL-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates or serum to each empty well. We recommend that each rat IL-4 standard solution and each sample is measured in duplicate.

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