SSPE Buffer, 20X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.
- 20X concentrated stock solution - DEPC Treated
- 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4
- Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers
The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Application Note: This product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water treated with diethyl pyrocarbonate (DEPC).
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