Human WISP1 - CCN4 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0701
76235-882EA 1127.31 USD
76235-882
Human WISP1 - CCN4 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human WISP1 - CCN4 AccuSignal ELISA Kit


  • Natural and recombinant human WISP1.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: T23-N367


WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human WISP1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human WISP1 standard solution and each sample be measured in duplicate.

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