Mouse ESM1 - Endocan ELISA Kit, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Mouse ESM1 - Endocan AccuSignal ELISA Kit
- Natural and recombinant mouse ESM1.
- There is no detectable cross-reactivity with other relevant proteins.
- Expression system for standard: NSO; Immunogen sequence: W20-R184
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1500pg/ml, 750pg/ml, 419pg/ml, 187.5pg/ml, 93.7pg/ml, 46.8pg/ml, 23.4pg/ml mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.
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