Anti-IL1R2 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemical
209-401-305-0100
76234-948EA 551.64 USD
76234-948
Anti-IL1R2 Rabbit Polyclonal Antibody
Antibodies
Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

This IgG fraction antibody of anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA,  radioimmunoassays, flow cytometry and immunoprecipitation.  Reactivity in other immunoassays is unknown.   IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.  This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay. 

Type: Primary
Antigen: IL1R2
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity:
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