Llama IgG Fluorescein Conjugate
- control or standard reagent
- multiplex, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting
Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35% - 70% of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa.
In llama and all other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies. All gamma chain camelid antibodies exhibiting the more conventional assembly of two light and two heavy chains with molecular weight ~150 kDa, belong to the IgG1 subclass.
Llama IgG Fluorescein can be utilized as a control or standard reagent in SDS, Western Blotting, Flow Cytometry, Immunofluorescence, and FLISA experiments. Specific conditions for reactivity should be optimized by the end user.
