NFκB p65 (Total/Phospho) ELISA kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
For the quantitative determination of active NFκB p65 in cell lysates of human, mouse, and rat origin.
The NFκB p65 EIA kit provides a sensitive, rapid and reliable method to measure the active form of NFκB p65. The assay uses streptavidin-coated plates with bound NFκB biotinylated-consensus sequence to capture only the active form of NFκB. The captured active NFκB is incubated with a specific NFκB p65 antibody, which is then detected using an HRP conjugated secondary antibody. The assay is developed with a chemiluminescent substrate and the signal is detected using a luminometer. Higher throughput 96 well plate based method faster than EMSA and Western blot with sensitive chemiluminescent detection.
- Fast- measure the active form of NFκB p65 in just 1.5 hours
- Higher throughput - 96 well plate based method faster than EMSA and Western blot
- Sensitive - colorimetric detection
Ordering information: Contains Microtiter plate, cell lysis buffer, 10X wash buffer, detection reagent, stop solution, plate sealer, capture antibody, detector antibody, positive control lysate.
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