E. coli Exonuclease III
Supplier: Biosearch Technologies
Digest duplex DNA in a 3´ to 5´ direction from a nick, blunt end, or 3´-recessed end, producing stretches of ssDNA
- Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends
- Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs
Exonuclease III digests duplex DNA in a 3´ to 5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible digestion results.
Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each template must be optimized using sample digestions to achieve the desired excision rate.
Exonuclease III is not active on 3´-protruding ends of four bases or more in length, ssDNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3´-DNA phosphatase, and apurinic DNA endonuclease activities.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include enzyme and a 10X reaction buffer.
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