Exonuclease VII
Supplier: Biosearch Technologies
ssDNA exonuclease with both 5´ to 3´ and 3´ to 5´ exonuclease activities
- Remove single-stranded oligonucleotide primers after PCR
- Minimize the effect of primers left over from previous PCR amplifications
- Heat inactivate the enzyme at 95°C for 10 minutes
Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´ to 3´ and 3´ to 5´ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95°C for 10 minutes.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include enzyme and a 5X reaction buffer.
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