DNase
Supplier: Biosearch Technologies
Effectively digest large or small dsDNA and ssDNA into mononucleotides
- Digest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers
- Use in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I
Applications include:
Removal of genomic DNA from small-sample total RNA preparations for expression analysis
Removal of small DNA oligonucleotides (e.g., random primers)
Even the small DNA oligonucleotides that remain after treatment with bovine pancreatic DNase I are undetectable by gel electrophoresis following treatment with Baseline-ZERO DNase. Removal of DNA from RNA preparations is particularly beneficial when RNA in a sample is amplified using a method that involves reverse transcription using random primers, since any contaminating DNA would also be a template for random-primed cDNA synthesis.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include enzyme and a 10X reaction buffer. Units reported are MBU or Molecular Biology Units. See the product user manual for the definition of MBU.
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