Nick translation DNA labeling system 2.0, Enzo Life Sciences

Supplier: Enzo Life Sciences

GEN111-0050
76013-836EA 380.41 USD
76013-836
Nick translation DNA labeling system 2.0, Enzo Life Sciences
Protein Linking and Labeling Systems

Nick translation system for preparing FISH probes


  • Ready-to-use Nick Translation Enzyme Mix minimizes pipetting error
  • Short labeling time - only 1 hour
  • Validated with fluorophore, biotin, and digoxigenin labeled dUTPs


The Nick Translation DNA Labeling System 2.0 provides a simple and efficient method for generating labeled DNA


The Nick Translation DNA Labeling System 2.0 can accommodate a wide range of fluorophore-labeled, biotin-labeled, and digoxigenin-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP. The ready-to-use NT Enzyme Mix is user friendly and minimizes error from pipetting.


Probes labeled by nick translation can be used in many different hybridization techniques including: in situ hybridization (ISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and reassociation kinetic studies.


Contains NT Enzyme Mix 250 µL, Reaction Buffer 250 µL, dNTP Mix (dATP, dGTP, dCTP) 250 µL, dTTP 180 µL, Stop Buffer 250 µL, Nuclease-free Water 5 mL.

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