Ubiquitin Activating Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

UW0400A-0001

76002-186EA 244.16 USD

76002-186

Ubiquitin Activating Kit, Enzo Life Sciences

Assays Enzyme Assays

Uses:

For use with wild type or mutant E2 enzymes in E1 initiated/mediated reactions
Contains highly sensitive ubiquitin antibody for detection of ubiquitin and ubiquitin conjugates

For activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilizing the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.

Ordering information: Contains 20X Ubiquitin Activating Enzyme Solution, Ubiquitin Solution, Ubiquitin Antibody Solution, Ubiquitin protein conjugates rabbit PAb, 20X Mg-ATP Solution, 2X Non-reducing Gel Loading Buffer, 10X Ubiquitinylation Buffer.

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