Lysine-C™, Sequencing Grade, G-Biosciences

Supplier: G-Biosciences

786-14

75998-072EA 521.37 USD

75998-072

Lysine-C™, Sequencing Grade, G-Biosciences

Nucleic Acid Reagents Sanger Sequencing Reagents

SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymatic activity.

SG-Lysine-C™ is a modified Lysine-C with high specificity, free from other known endopeptidases, that is resistant to autolysis and degradation

SG-Lysine-C™ is supplied lyophilized in 5 μg vials. The enzyme is typically reconstituted to a concentration of 0.25 μg/mL. For fragmentation, the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight) in a standard digestion buffer.

SG-Chymotrypsin™ is used for the digestion of proteins for sequence analysis and is suitable for sequencing applications. Use of SG-Lysine-C™ produces consistent activity levels from lot to lot for reproducible digestions.

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