HeLa Cell Nuclear Extract Etoposide Stimulated, Rockland Immunochemicals
Supplier: Rockland Immunochemical
HeLa Cell Nuclear Extract Etoposide Stimulated
- Ready-to-use lysates
- positive controls
- treated lysated
- nuclear lysates
Ready-to-use HeLa Cell Nuclear Extract Etoposide Stimulated produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 ul depending on the size format of your gel.
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