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EnzyChrom™ Lactate Assay Kit, BioAssay Systems

Supplier: BIOASSAY SYSTEMS

EnzyChrom™ Lactate Assay Kit, BioAssay Systems

EnzyChrom™ Lactate Assay Kit, BioAssay Systems

ECLC-100

75878-070EA 667.1 USD

75878-070

EnzyChrom™ Lactate Assay Kit, BioAssay Systems

Assays Cellular Assays

For quantitative colorimertic determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

Linear detection range 0.05 - 2 mM L-Lactate in 96-well plate assay
Convenient room temperature assay. No 37°C heater is needed
Can be readily automated to process thousands of samples per day

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready. Procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

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