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Cell Stimulation and Protein Transport Inhibitor Cocktail, Biogems

Supplier: BIOGEMS INTERNATIONAL INC.

63510-00-100

75843-426EA 117.54 USD

75843-426

Cell Stimulation and Protein Transport Inhibitor Cocktail, Biogems

Assays Cellular Assays

The cell stimulation and protein transport inhibitor cocktail is a pre-mixed cocktail used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection.

The solution is composed of an optimized mixture of brefeldin A, ionomycin, monensin, and PMA. PMA and ionomycin induce cytokine production in many cell types and brefeldin A and monensin are protein transport inhibitors.

The cell stimulation and protein transport inhibitor cocktail is supplied at a 500X concentration and should be used at 1X final concentration for cell stimulation. It can be added directly to the cell culture medium at 2 µl/ml. It has been tested by the stimulation of mouse splenocytes and subsequent intracellular staining and flow cytometry analysis of IFN-gamma.

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