Corning® HAT (Hypoxanthine, Aminopterin, Thymidine), Corning

Supplier: Corning

25-046-CI
45000-686EA 93.11 USD
45000-686
Corning® HAT (Hypoxanthine, Aminopterin, Thymidine), Corning
Cell Culture Supplements and Additives

HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells


The combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor, with hyproxanthine and thymidine, which are intermediates in DNA synthesis, provides a form of artificial selection for cells containing functioning hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and thymidine kinase (TK). These enzymes are involved in DNA synthesis and cell division. HAT medium is often used in the preparation of monoclonal antibodies.

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