Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
Recommended Dilutions: ELISA: 1:10,000-1:20,000; Western Blot: 1 - 2 µg/mL; contains 0.02% (w/v) Sodium Azide
Type: Primary
Antigen: CDC23
Clonality: Polyclonal
Clone: 0
Conjugation: Unconjugated
Epitope: Center
Host: Rabbit
Isotype:
Reactivity: