CXCR4 antibody detects a G coupled -protein receptor. Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. Anti-CXCR4 antibodies are ideal for investigators involved in Cytokines and Growth Factors or Infectious Disease reasearch.
Recommended Dilutions: Flow Cytometry: 1:50-1:100; Immunohistochemsitry: 10 ug/ml; Immunofluorescence Microscopy: 1:100; Immunoprecipitation: 10 ug/ml; Western Blot: 1-2 ug/mL; contains 0.01% (w/v) Sodium Azide
Type: Primary
Antigen: CX3CR1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: N-Terminal
Host: Rabbit
Isotype:
Reactivity:
