Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Recommended Dilutions: ELISA: 1:20,000-1:40,000; Immunohistochemsitry: 10 ?g/mL; Immunofluorescence Microscopy: 20 ?g/mL; Western Blot: 2-4 ?g/mL; contains 0.02% (w/v) Sodium Azide
Type: Primary
Antigen: BUB1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: N-Terminal
Host: Rabbit
Isotype:
Reactivity: Human, Mouse
