Anti-AES Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemical
600-401-X76
10799-580EA 635.22 USD
10799-580
Anti-AES Rabbit Polyclonal Antibody
Antibodies
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Recommended Dilutions: ELISA: 1:20,000-1:40,000; Immunofluorescence Microscopy: 20 ?g/mL; Western Blot: 2 - 4 µg/mL; contains 0.02% (w/v) Sodium Azide

Type: Primary
Antigen: AES
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: N-Terminal
Host: Rabbit
Isotype:
Reactivity: Human, Mouse
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