Electrocompetent Agrobacterium Combo Pack, Intact Genomics

Supplier: INTACT GENOMICS, INC MS
1290-24
104013-318PK 865.38 USD
104013-318
Electrocompetent Agrobacterium Combo Pack, Intact Genomics
Competent Cells
Intact Genomics electrocompetent Agrobacterium combo pack is perfect for scientists who require multiple Agrobacterium competent cell strains for their research. The electrocompetent Agrobacterium combo pack contains three 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.

  • High transformation efficiencies
  • Especially useful for cDNA gDNA library construction
  • Convenience of multiple strains for researching different plants

The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for the transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn. The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection.

AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for the transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.  LBA4404 contains a rifampicin resistance gene (rif).  LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

Delivery information: All our products are shipped with dry ice via FedEx overnight delivery. US orders received by 1:00 pm (Central time, Monday-Thursday) will typically be shipped on the same day in the US. Orders after 1:00 PM will be shipped on the next business day. Orders received on Friday will be shipped on the following Monday.
The shipping and dry ice packaging cost is $45 for your entire order in the 48 contiguous United States.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR