Biotin (Serum/Plasma/Urine) ELISA Assay Kit, Eagle Biosciences, Inc.

Supplier: Eagle Biosciences

KR8141
103389-274EA 1425.38 USD
103389-274
Biotin (Serum/Plasma/Urine) ELISA Assay Kit, Eagle Biosciences, Inc.
Assays ELISAs

Biotin (Serum/Plasma/Urine) ELISA is intended to detect biotin in serum, plasma, urine and milk.


  • Dynamic Range: 48.1 - 1100 ng/L
  • Sensitivity: 32.4 ng/L
  • Incubation: 1.25 hours


Biotin (vitamin H) is present in bacterial, funghi, plants and animals. In food, the major part of biotin is covalently bound to protein, leaving only a minor fraction freely available. During digestion, biocytin (biotinyl-lysine) is released from the proteins and can be resorbed as easily as biotin from the intestinal tract. Afterwards, biotin is released from biocytin by the enzyme biocytinase in erythrocytes and plasma. It is then available as prosthetic group for a series of biotin-dependent enzymes. The daily requirements of biotin are difficult to estimate because a healthy intestinal flora supplies a major part of biotin by endogenous synthesis. The generally recommended daily dose for adults is 100–200 μg. Chronic hemodialysis patients show clear improvements of neuropathological status and glucose metabolism when supplemented with biotin in a milligram range. Biotin deficiency can be caused by e. g. destruction of the intestinal flora or extreme diets (e. g. frequent consumption of raw eggs). It can lead to dermatitis, hair loss, anorexia, mucular hypotonia, depression and reproduction problems.

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