RtcB Ligase
Supplier: New England Biolabs (NEB)
M0458S
103307-256EA
87
USD
103307-256
RtcB Ligase
Nucleic Acid Reagents
Cloning Kits and Ligation
RtcB Ligase is expressed as a His-tagged fusion in E. coli.
- This is an Enzyme for Innovation (EFI)
- These enzymes have interesting properties and unique specificities
RtcB Ligase from E. coli joins single stranded RNA with a 3'-phosphate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl. Ligation requires both GTP and MnCl₂ and proceeds through a 3'-guanylate intermediate. For substrates with a 2',3'-cyclic phosphate end, hydrolysis to a 3'-phosphate precedes 3' end activation with GMP and ligation.
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