IGF-I ELISA , Eagle Biosciences
Supplier: Eagle Biosciences
Human Insulin-like Growth Factor-1 (IGF-1) ELISA determines IGF-1 in serum
- Dynamic Range: 2 - 50 ng/ml
- Sensitivity: 0.11 ng/mL
- Incubation: 1.75 hours
Insulin-like growth factors (IGF) -I and -II play a pivotal role in regulating the proliferation, differentiation and specific functions of many cell types (1-3). IGF-I is identical with Somatomedin C (Sm-C) (4) and has a molecular weight of 7649 daltons (5). Its major regulators are growth hormone (GH) and nutrition (6), although its production in specific tissues is affected by a multitude of tropic hormones and other peptide growth factors. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven classes of IGFBPs which are known at present (7,8,22) either bind IGF-I and IGF-II with similar affinities or show a preference for IGF-II (9,10).A major problem of IGF-I measurement results from the interference of IGFBPs in the assay. Direct determinations in untreated serum samples (11) give false values because of the extremely slow dissociation of the IGF-I/IGFBP-3 complexes during the assay incubation. Depending on the ratio IGF-I to IGFBP the following errors may occur.
For Research Use Only.
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