NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly Cloning Kit - 10 rxns

NEBuilder HiFi DNA Assembly Cloning Kit - 10 rxns

E5520S

102877-808EA 212 USD

102877-808

NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Nucleic Acid Reagents Cloning Kits and Ligation

The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Elute in as little as 6 μL
Eliminate buffer retention and salt carryover with optimized column design
Save time with fast, user-friendly protocols
No need to monitor the pH of your reaction
Reduce your impact on the environment
Use less plastic
Responsibly-sourced, recyclable packaging
Quickly purify DNA from agarose gels

It was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.

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