TRI CAT ELISA

Supplier: ALPCO DIAGNOSTICS MS

17-TCTHU-E03.1

102672-628EA 2290.77 USD

102672-628

TRI CAT ELISA

Assays ELISAs

The tri-CAT ELISA (epinephrine, norepinephrine and dopamine) is for the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), and dopamine in plasma and urine.

Each catecholamine is measured independently on separate plates

Adrenaline (epinephrine), noradrenaline (norepinephrine), and dopamine are extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.

Caution: For research use only. Not for use in diagnostic procedures.

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