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Q5® High-Fidelity DNA Polymerase

Supplier: New England Biolabs (NEB)

Q5 High-Fidelity DNA Polymerase - 100 units

Q5 High-Fidelity DNA Polymerase - 100 units

M0491L M0491S

102500-136EA 528 USD

102500-136 102500-138

Q5® High-Fidelity DNA Polymerase

Nucleic Acid Reagents End-point PCR Enzymes and Kits

Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

Highest fidelity amplification (~280X higher than Taq)
Ultra-low error rates
Superior performance for a broad range of amplicons (from high AT to high GC)
Hot start and master mix formats available

With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.

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