Q5® High-Fidelity 2X Master Mix

Supplier: New England Biolabs (NEB)

M0492L M0492S
102500-132EA 868 USD
102500-132 102500-134
Q5® High-Fidelity 2X Master Mix
Nucleic Acid Reagents End-point PCR Enzymes and Kits

The Q5 High-fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format.



  • Highest fidelity amplification (~280X higher than Taq)

  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available


The Q5 High- fidelity 2X master mix features a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.


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