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Histamine Research ELISA

Supplier: ALPCO DIAGNOSTICS MS

17HISRTE01RES

101721-022EA 1118.46 USD

101721-022

Histamine Research ELISA

Assays ELISAs

The research histamine ELISA is for the quantitative determination of Histamine in EDTA plasma of various animal species.

The research histamine ELISA is suitable for animal species including mouse, rat, equine, canine, and cat

Enzyme immunoassay for the quantitative determination of histamine in different animal species and biological fluids. During the sample preparation, histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls, and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen, and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using
TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Caution: For research use only. Not for use in diagnostic procedures.

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