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ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

Supplier: BIO-RAD LABORATORIES MS

ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

1632089

101644-026EA 986.15 USD

101644-026

ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

Protein Extraction Protein Extraction Kits

The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

The ReadyPrep protein extraction kit (cytoplasmic/nuclear):

* Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989)
* Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins
* Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that may interfere with IEF

* Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei (Dignam et al

References
- Dignam JD et al. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acid Res 11, 1475–1489.

- Zerivitz K and Akusjarvi G (1989). An improved nuclear extract preparation method. Gene Anal Tech 6, 101–109.

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