PUREXPRESS® RF123 Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
Release factors are involved in termination of protein translation by recognizing the stop codons in an mRNA sequence
In a ribosome display experiment using PURExpress, lack of release factors could stabilize the ternary complex of mRNA-ribosome-nascent protein. As a result, the cDNA recovery could be higher. In this kit, the three release factors are supplied separately, allowing users to perform a protein synthesis reaction/ribosome display experiment with/without release factors of their choice.
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