PUREXPRESS® Disulfide Bond Enhancer, New England Biolabs
Supplier: New England Biolabs (NEB)
The PURExpress® Disulfide Bond Enhancer (PDBE) is a proprietary blend of proteins and buffer components designed to correctly fold target proteins with multiple disulfide bonds produced in PURExpress reactions or E.coli S30 extracts.
Added at the beginning of a reaction, the components promote a proper disulfide bond pattern by assisting with the oxidation of cysteine thiols and correcting mis-oxidized substrates. These enhancements can increase the yield of soluble and functionally active protein.
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