VWR® DNase, Double-Strand Specific, Heat-Labile

Supplier: VWR

DNase, Double-Strand Specific, Heat-Labile treatment leaves RNA quality intact. RNA incubated with buffer, water or DNase were analyzed using the Eukaryote Total RNA StdSens Assay (Bio-Rad Experion System), with the results indicating all samples had intact, high quality RNA (RQI <gt/>8.5)

A PCR master mix containing 50 ng genomic DNA was prepared and divided into two reactions, of which one was treated with DNase, Double-Strand Specific, Heat-Labile prior to qPCR of both samples. In contrast to the untreated sample, the DNase-treated sample contained no amplifiable genomic DNA.

VWR® DNase, Double-Strand Specific, Heat-Labile

VWR® DNase, Double-Strand Specific, Heat-Labile

DNase, Double-Strand Specific, Heat-Labile treatment leaves RNA quality intact. RNA incubated with buffer, water or DNase were analyzed using the Eukaryote Total RNA StdSens Assay (Bio-Rad Experion System), with the results indicating all samples had intact, high quality RNA (RQI <gt/>8.5)

A PCR master mix containing 50 ng genomic DNA was prepared and divided into two reactions, of which one was treated with DNase, Double-Strand Specific, Heat-Labile prior to qPCR of both samples. In contrast to the untreated sample, the DNase-treated sample contained no amplifiable genomic DNA.

VWR® DNase, Double-Strand Specific, Heat-Labile

VWR® DNase, Double-Strand Specific, Heat-Labile

1B1662-250U

10147-174EA 684.27 USD

10147-174

VWR® DNase, Double-Strand Specific, Heat-Labile

Enzymes

VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 – 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini

High specificity for dsDNA, while leaving ssDNA and RNA intact
Heat inactivates completely and irreversibly at 55°C
Ideal for genomic DNA removal in RNA preps
Removes contaminating dsDNA from PCR master mixes

Store frozen (-20 – 0°C). Stable two years when stored frozen (-20 – 0°C) and up to six months when stored cold (2 – 8°C). The enzyme tolerates multiple freeze-thaw cycles.

The high specific activity of this enzyme toward dsDNA can be inactivated by heating at 55°C, conveniently eliminating the need for its physical or chemical removal before downstream processing, even in the presence of RNA and ssDNA, such as primers and probes. DNase, Double-Strand Specific, Heat-Labile is ideal for removal of genomic DNA in RNA preps and for removal of DNA carryover contamination in PCR mixes, before addition of template.

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